合成生物学与生物技术专家。深圳理工大学合成生物学院院长、讲席教授，中国科学院生物物理所研究员，中国科学院武汉病毒研究所荣誉研究员，加拿大Alberta大学荣誉科学博士，美国医学与生物工程院院士、英国皇家化学会会士。湖北大学毕业，分别在中国科学院武汉病毒研究所和中国科学院微生物研究所获硕士和博士学位。从事合成生物学、生物传感、纳米生物学和分析微生物学交叉创新研究，并用于解决细胞生物学、病毒学、肿瘤生物学的基础科学问题。发表研究论文400多篇、学术专著3本、获专利40余项。

1. AI与机器学习的运用：肿瘤细胞分子影像分析、急性髓系白血病连续分级异质性与预后评估、新型抗菌肽预测、非小细胞肺癌细胞外囊泡生物标志物的发现等，肿瘤诊断-治疗体系设计与合成，微生物底盘细胞代谢重构。

2. 基因编码/合成生物传感器：可视化研究活细胞内分子事件，如生物分子相互作用与相变、生物膜生长、亚细胞结构对环境刺激的响应、细胞凋亡、细胞核染色质群体空间分布、肿瘤单细胞表面抗原分析等，超高灵敏度病毒检快速检测。

3. 病毒宿主互作示踪：设计构建“彩色病毒”，实现病毒-宿主细胞互作高时空分辨动态示踪，发现和阐明了病毒（HIV、新冠病毒、流感病毒、寨卡病毒等）侵染及生活史中新的机制（如HIV病毒粒子通过核膜内吞方式入核等），丰富了病毒学知识。

4. 蛋白组芯片：创建结核分枝杆菌全蛋白组芯片及蛋白质组库、基因敲除库、成为结核病研究的高通量、高内涵平台，发现一批新的免疫原，其衍生产品免疫试剂盒已广泛用于结核病防控和结核病研究。创建非洲猪瘟全蛋白组芯片，筛选免疫原，用于新型疫苗研究。

5. 纳米生物学：以病毒和细菌组件、细胞外泌体、微生物酶、干细胞等天然纳米生物材料为分子元件，设计合成多层级结构生物纳米功能器件，用于构建纳米生物传感器、体内药物递送载体、生物反应过程生化参数检测与控制系统、环境有机污染物监测等。

宏观战略研究：

担任国家合成生物学重点专项实施方案和指南专家组组长；中国合成生物学发展路线图（2022-2030）研究组组长；中科院十五五生物技术发展战略研究专家组组长；《国家中长期科学和技术发展规划(2021—2035)》基础科学战略研究专家组副组长及交叉科学组组长（2019）；亚太经合组织（APEC）首席科学顾问会议中方代表（CSAE,2013,2015-2018）。在生命科学、合成生物学、生物技术和生物制造领域承担大量国家层次战略研究任务。

学术组织任职：

目前担任：中国生物工程学会副理事长、合成生物学分会主任、生物传感/生物芯片/纳米生物技术分会创会主任、荣誉主任；亚洲生物技术协会（AFOB）顾问、纳米生物技术/生物传感/生物芯片分会共同创会长；世界生物传感学术大会（World Congress on Biosensors）常务组委会委员；中国生物技术研究中心专家委员会委员。

Biosensors & Bioelectronics、mLife、中国科学院院刊等10种期刊编委，《合成生物学丛书》（科学出版社）总主编，《中华科学技术大词典》编委会委员。

曾经担任：伦敦纳米中心（LCN）顾问组成员（2011-2016），中国微生物学会副理事长（2008-2016），中国生物物理学会副理事长（2009-2013），湖北省微生物学会理事长（2001-2009），全国科学技术名词审定委员会常委（2004-2015）；CFDA第一届医疗器械分类技术委员会临床检验专业组委员（2017-2022）。

所获荣誉：

1. 首届中科院青年科学家奖（1989）

2. 中科院有突出贡献中青年专家（1994）

3. 加拿大Alberta大学荣誉科学博士学位（2015）

4. 英国皇家化学会Fellow（2018）

5. 美国医学与生物工程院Fellow（2020）

6. 全国科技系统抗击新冠肺炎疫情先进个人（2021）

7. 科研管理岗位上获2次三等功（2007，2010）

8. 宏观战略论文两次获评《中国科学院院刊》年度十大热点论文：

• 世界生命科学格局中的中国，2022

• 解读新时代中国生物技术发展图景，2023

研究论文总共400余篇(论文目录可参考 Google Scholar：https://scholar.google.com/citations?hl=zh-CN&user=s5StRroAAAAJ)，学术专著3部，科技管理和战略发展论著4部。

代表性论著报告：

1. 生物传感器，化学工业出版社，2005

2. 合成生物学路线图2030，研究组长，科学出版社，2024

3. 合成生物学丛书（共12册），总主编，科学出版社，2024

4. 科技创新与强国之路，主编，化学工业出版社，2010

近5年研究论文：

1. Yuan Y, Jiang H, Xue R, Feng XJ, Liu BF, Li L, Peng B, Ren CS, Li SM, Li N, Li M, Wang DB, Zhang XE. Identification of a Biomarker Panel in Extracellular Vesicles Derived From Non-Small Cell Lung Cancer (NSCLC) Through Proteomic Analysis and Machine Learning. J Extracell Vesicles. 2025 May;14(5):e70078. doi: 10.1002/jev2.70078.

2. Wei Zhou, Shaojie Ma, Ruimin Gao, Yufu Tang, Hui Zhang, Ao Liang, Mengsi Yang, Chun Ma, Quli Fan, Xian-En Zhang, and Feng Li. Assembly of Matryoshka-Type Protein Nanocages for Compartmentalized Oxygen Sensing. Nano Letters 2025 25 (11), 4433-4440. doi: 10.1021/acs.nanolett.4c06699.

3. Lu Xiao, Xuexi Wang, Dujuan Liu, Chuang Yan, Xian-En Zhang, and Minghai Chen. Genetically Encoded Single-Wavelength Sensor with High Specificity for Imaging ATP in Living Cells. ACS Sensors 2025 10 (2), 1398-1406. doi: 10.1021/acssensors.4c03389.

4. Yang C, Tang J, Sun J, Liu Z, Zhang G, Li Z, Wang DB, Zhang XE. Ultrasensitive detection of miRNA with single-nucleotide resolution using a high-electron-mobility transistor biosensor combined with splint-ligation. Biosens Bioelectron. 2025 Jul 2;287:117743. doi: 10.1016/j.bios.2025.117743.

5. Ying Zheng, Chunyang Zhang, Ying Zhang, Kun Zhou, Pengwei Tan, Youjiang Liu, Guotao Duan, Hengfei Li, Chilai Chen, Cunlan Guo, Xiaodong Li, Chen Chen, Ashaq Ali, Yang Zhang, Xian-En Zhang, and Dong Men. A Nanomaterial-Independent Biosensor Based on Gallium Arsenide High-Electron-Mobility Transistors for Rapid and Ultra-Sensitive Pathogen Detection. ACS Sensors 2025 10 (6), 3898-3908. doi: 10.1021/acssensors.4c03134.

6. Wang L, Li SM, Li X, Wu HS, Ma C, Wang DB, Zhang XE. The transmembrane nucleoporin Nup210 weakens HIV-1 infection via modulating late events of viral nuclear import. AIDS. 2025 Aug 1;39(10):1322-1330. doi: 10.1097/QAD.0000000000004206.

7. Yang C, Sun J, Zhang Y, Tang J, Liu Z, Zhan T, Wang DB, Zhang G, Liu Z, Zhang XE. Construction of AlGaN/GaN high-electron-mobility transistor-based biosensor for ultrasensitive detection of SARS-CoV-2 spike proteins and virions. Biosens Bioelectron. 2024 Aug 1;257:116171. doi: 10.1016/j.bios.2024.116171.

8. Liu Q, Wang D, Cui M, Li M, Zhang XE. A genetically encoded fluorescent protein sensor for mitochondrial membrane damage detection. Biochem Biophys Res Commun. 2024 May 21;709:149836. doi: 10.1016/j.bbrc.2024.149836.

9. Wang XF, Tang JY, Sun J, Dorje S, Sun TQ, Peng B, Ji XW, Li Z, Zhang XE, Wang DB. ProT-Diff: A Modularized and Efficient Strategy for De Novo Generation of Antimicrobial Peptide Sequences by Integrating Protein Language and Diffusion Models. Adv Sci (Weinh). 2024 Nov;11(43):e2406305. doi: 10.1002/advs.202406305.

10. Shao N, Ren C, Hu T, Wang D, Zhu X, Li M, Cheng T, Zhang Y, Zhang XE. Detection of continuous hierarchical heterogeneity by single-cell surface antigen analysis in the prognosis evaluation of acute myeloid leukaemia. BMC Bioinformatics. 2023 Nov 28;24(1):450. doi: 10.1186/s12859-023-05561-0.

11. Xiayi Feng, Ding Gao, Juan Zhou, Zhu Gui, Aoling Cai, Jie Wang, Ji Dai, Yipeng Jing, Junchao Qian, Zongqiang Cui, Lanxiang Li, Yue You, Luyi Tian, Xian-En Zhang, Dong Men. Viral capsid-mediated efficient cell labeling of micron-sized magnetic particles for highly sensitive long-term in vivo tumor cells MRI tracking. Chem. Engin. J. 2024 498(155830), 1385-8947. doi: 10.1016/j.cej.2024.155830.

12. Yu JF, Xu JT, Feng A, Qi BL, Gu J, Deng JY, Zhang XE. Competition between H4PteGlu and H2PtePAS Confers para-Aminosalicylic Acid Resistance in Mycobacterium tuberculosis. Antibiotics (Basel). 2023 Dec 21;13(1):13. doi: 10.3390/antibiotics13010013.

13. Wang K, Pan Y, Wang D, Yuan Y, Li M, Chen Y, Bi L, Zhang XE. Altered hACE2 binding affinity and S1/S2 cleavage efficiency of SARS-CoV-2 spike protein mutants affect viral cell entry. Virol Sin. 2023 Aug;38(4):595-605. doi: 10.1016/j.virs.2023.06.005.

14. Lu S, Hou Y, Zhang XE, Gao Y. Live cell imaging of DNA and RNA with fluorescent signal amplification and background reduction techniques. Front Cell Dev Biol. 2023 Jun 5;11:1216232. doi: 10.3389/fcell.2023.1216232.

15. Song L, Wang D, Abbas G, Li M, Cui M, Wang J, Lin Z, Zhang XE. The main protease of SARS-CoV-2 cleaves histone deacetylases and DCP1A, attenuating the immune defense of the interferon-stimulated genes. J Biol Chem. 2023 Mar;299(3):102990. doi: 10.1016/j.jbc.2023.102990.

16. Zhang XE, Liu C, Dai J, Yuan Y, Gao C, Feng Y, Wu B, Wei P, You C, Wang X, Si T. Enabling technology and core theory of synthetic biology. Sci China Life Sci. 2023 Aug;66(8):1742-1785. doi: 10.1007/s11427-022-2214-2.

17. Abbas G, Cui M, Wang D, Li M, Zhang XE. Construction of Genetically Encoded Biosensors to Monitor Subcellular Compartment-Specific Glutathione Response to Chemotherapeutic Drugs in Acute Myeloid Leukemia Cells. Anal Chem. 2023 Feb 7;95(5):2838-2847. doi: 10.1021/acs.analchem.2c04255.

18. Zheng L, Wang K, Chen M, Qin F, Yan C, Zhang XE. Characterization and Function of Glycans on the Spike Proteins of SARS-CoV-2 Variants of Concern. Microbiol Spectr. 2022 Dec 21;10(6):e0312022. doi: 10.1128/spectrum.03120-22.

19. Feng X, Gao D, Jing Y, Qian J, Cui Z, Zhou J, Zhang XE, Men D. Intracellular Delivery of Micron-Sized Magnetic Particles through a Virus Infection Pathway. ACS Appl Mater Interfaces. 2022 Oct 19;14(41):46850-46856. doi: 10.1021/acsami.2c11991.

20. Chen M, Yan C, Qin F, Zhang XE. Near-Infrared Luciferase Complementation Assay with Enhanced Bioluminescence for Studying Protein-Protein Interactions and Drug Evaluation Under Physiological Conditions. Anal Chem. 2022 Oct 11;94(40):13700-13709. doi: 10.1021/acs.analchem.2c01238.

21. Su W, Qiu J, Mei Y, Zhang XE, He Y, Li F. A microfluidic cell chip for virus isolation via rapid screening for permissive cells. Virol Sin. 2022 Aug;37(4):547-557. doi: 10.1016/j.virs.2022.04.011.

22. Gong R, Yang C, Abbas G, Wang D, Zhang XE, Nie G, Ding H. Diagnosis of nasopharyngeal carcinoma using an ultrasensitive immunoassay method based on nanoparticles. Nanoscale. 2023 Feb 16;15(7):3475-3481. doi: 10.1039/d2nr05848k.

23. Tang J, Zhang G, Li F, Zeng R, Song J, Abbas G, Cui M, Zhang W, Zhang XE, Wang DB. Two-Dimensional Protein Nanoarray as a Carrier of Sensing Elements for Gold-Based Immunosensing Systems. Anal Chem. 2022 Jul 5;94(26):9355-9362. doi: 10.1021/acs.analchem.2c01190.

24. Hou Y, Wang D, Lu S, Guo D, Li M, Cui M, Zhang XE. Optogenetic Control of Background Fluorescence Reduction for CRISPR-Based Genome Imaging. Anal Chem. 2022 Jun 21;94(24):8724-8731. doi: 10.1021/acs.analchem.2c01113.

25. Lin R, Xiao M, Cao S, Sun Y, Zhao L, Mao X, Chen P, Tong X, Ou Z, Zhu H, Men D, Li X, Deng Y, Zhang XE, Wen J. Distinct gut microbiota and health outcomes in asymptomatic infection, viral nucleic acid test re-positive, and convalescent COVID-19 cases. mLife. 2022 Jun;1(2):183-197. doi: 10.1002/mlf2.12022.

26. Ma Y, Mao G, Yang W, Wu G, Li G, Li X, Lin X, Lu J, Zhao S, Zhao W, Dai J, Zhang XE. Studying bona fide SARS-CoV-2 biology in a BSL-2 biosafety environment using a split-virus-genome system. Sci China Life Sci. 2022 Sep;65(9):1894-1897. doi: 10.1007/s11427-022-2114-8.

27. Mao G, Wu G, Chen M, Yan C, Tang J, Ma Y, Zhang XE. Synthesis of Dual-Emitting CdZnSe/Mn:ZnS Quantum Dots for Sensing the pH Change in Live Cells. Anal Chem. 2022 May 10;94(18):6665-6671. doi: 10.1021/acs.analchem.

28. Li S, Wang D, Ghulam A, Li X, Li M, Li Q, Ma Y, Wang L, Wu H, Cui Z, Zhang XE. Tracking the Replication-Competent Zika Virus with Tetracysteine-Tagged Capsid Protein in Living Cells. J Virol. 2022 Apr 13;96(7):e0184621. doi: 10.1128/jvi.01846-21.

29. Cui, M., Abbas, G., Wang, D., Liu, Q., Gong, R., Li, M., & Xian-En Zhang. Genetically encoded redox biosensor system for H2O2 measurement in four subcellular compartments in acute myeloid leukemia (AML) cells. Science China. Life Sciences. 2022, 65 (6):1259-1262. doi: 10.1007/s11427-021-2049-6

30. Gao R, Tan H, Li S, Ma S, Tang Y, Zhang K, Zhang Z, Fan Q, Yang J, Zhang XE, Li F. A prototype protein nanocage minimized from carboxysomes with gated oxygen permeability. Proc Natl Acad Sci U S A. 2022 Feb 1;119(5):e2104964119. doi: 10.1073/pnas.2104964119.

31. Ma Y, Mao G, Wu G, Zhang XE. Single-Particle Tracking Reveals the Interplay between HIV-1 Reverse Transcription and Uncoating. Anal Chem. 2022 Feb 8;94(5):2648-2654. doi: 10.1021/acs.analchem.1c05199.

32. Jin Song, Yiwei Li, Dingkun Ke, Dianbing Wang, Xian-En Zhang. In situ graphene-modified carbon microelectrode array biosensor for biofilm impedance analysis. Electrochimica Acta. 2022 403(139570), 0013-4686. doi: 10.1016/j.electacta.2021.139570.

33. Cao S, Zhang Q, Song L, Xiao M, Chen Y, Wang D, Li M, Hu J, Lin L, Zheng Y, Zhou K, Ye S, Zhou J, Zhou YN, Cui J, Wang J, Sun J, Tao J, Chen Z, Chen R, Zhou P, Shi Z, Wei S, Zhao L, Wang H, Tong X, Li X, Men D, Hou B, Zhang XE. Dysregulation of Innate and Adaptive Immune Responses in Asymptomatic SARS-CoV-2 Infection with Delayed Viral Clearance. Int J Biol Sci. 2022 Jul 11;18(12):4648-4657. doi: 10.7150/ijbs.72963.

34. Gong R, Wang D, Abbas G, Li S, Liu Q, Cui M, Zhang XE. A switch-on molecular biosensor for detection of caspase-3 and imaging of apoptosis of cells. Sci China Life Sci. 2022 Mar;65(3):540-549. doi: 10.1007/s11427-021-1986-7.

35. Chen M, Yan C, Zheng L, Zhang XE. The smallest near-infrared fluorescence complementation system for imaging protein-protein and RNA-protein interactions. Chem Sci. 2021 Dec 25;13(4):1119-1129. doi: 10.1039/d1sc04839b.